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MBC 3 2025 - English

ASSIGNMENT

BIOANALYTICAL TECHNIQUES

Course Code: MBC-003

Assignment code: MBC-003/TMA/2025

Maximum marks: 100

Note: Attempt all questions. Write the answers in your own words; do not copy from the course material.

1. a) Define the following:

i) pH

ii) Molarity

iii) Numerical aperture

iv) Chemical fixation

v) Rotor

b) Explain the UV-Vis spectrophotometer with suitable diagram.

2. a) What is sub-cellular fractionation? Discuss its process.

b) Write the working principle and basic components of Confocal microscopy.

3. a) Discuss basic theory and application of Atomic force Microscopy.

b) Explain Raman spectroscopy and its applications.

4. a) Define Good Laboratory safety rules. Enlist the chemical safety instructions?

b) Write a brief short note on physical methods of cell disruption.

5. (a) How would you prepare the following laboratory solutions?

i) 250 ml of 0.2 M NaOH

ii) 50 ml of 0.5 N of HCl

b) Explain Bragg's Law of Diffraction with well labeled diagram.

6. Differentiate between:

a) Paper chromatography and thin layer chromatography

b) FISH and GISH techniques

7. a) Describe isoelectric focusing technique.

b) Discuss the western blotting technique.

8. Describe the basic theory, components and applications of the Gas chromatography.

9. a) Describe the Autoradiography

10. Explain the principle and applications of the following:

a) High-Performance Thin-Layer Chromatography

b) Liquid Chromatography-Mass Spectrometry

MBC 003 (January 2026 - July 2026) - ENGLISH

ASSIGNMENT

BIOANALYTICAL TECHNIQUES

Course Code: MBC-003

Assignment code: MBC-003/TMA/2026

Maximum marks: 100

Note: Attempt all questions. Write the answers in your own words; do not copy from the course material.

1. a) Define the following:

i) Mole fraction

ii) Stocks shift

iii) Chromatogram

iv) Half life of radioisotopes

v) Resolving power

b) Discuss the ionization of weak electrolytes with suitable example.

2. a) What is density gradient centrifugation? Discuss briefly any of its method.

b) Write the basic principle and application of NMR Spectroscopy.

3. a) Compare the ultrasonication and freeze thaw methods.

b) Write the basic components and application of Fluorescence spectrophtometry.

4. a) What are Good Laboratory safety rules. Why they are important?

b) Write a brief account note on Bioluminescence and its use in microscopy.

5. (a) How would you prepare the following laboratory solutions? 

i) 500 ml of 0.5 M Na₂CO₃

ii) 100 ml of 0.1 N of NaOH

b) Elaborate the different application of electron microscopy.

6. Differentiate between:

a) Gas Chromatography and HPLC

b) Southern Blotting and Northern blotting

7. a) Describe SDS-PAGE technique.

b) Write an overview on Biological Hazard and its safety aspects.

8. Describe the basic theory, components and applications of thin layer chromatography.

9. a) Explain the steps and application of FISH technique with suitable diagram.

10. Write short notes on the following:

a) Major types of ion sources of mass spectrometer.

b) Ultra-Performance Liquid Chromatography (UPLC)

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