IGNOU MBC 4 SOLVED ASSIGNMENT

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MBC 4 2025 - English

ASSIGNMENT

BIOANALYTICAL TECHNIQUES

Course Code: MBC-004

Assignment code: MBC-004/TMA/2025

Maximum marks: 100

Note: Attempt all questions. The marks for each question are indicated against it.

Write the answers in your own words; do not copy from the course material.

PART-(A)

1. a) Distinguish between :

i) Enzyme Activity and Specific Enzyme Activity

ii) Isomerases and Transferases

b) Explain the effect of pH on enzyme activity.

2. Discuss different types of enzyme assays.

3. a) What are metal activated enzymes?

b) Explain strain and distortion mechanism of catalysis

4. Derive and draw the following:

i) Hanes-Woolf Plot

ii) Eadie-Hofstee Plot

5. Derive Michaelis Menten equation for a mixed inhibitor.

PART- (B)

6. What are allosteric enzymes? Distinguish between MWC and KNF model

7. a) Describe the methods based on polarity of enzyme purification.

b) Write short note on Taq Polymerase.

8. Give an overview of Fatty Acyl multienzyme complex.

9. Describe application of enzymes in the following:

a) Starch industry

b) Wine industry

10. a) Explain the properties of Immobilized enzymes.

b) Describe the rational protein design of enzyme engineering.

MBC 004 (January 2026 - July 2026) - ENGLISH

ASSIGNMENT

ENZYMES AND THEIR APPLICATIONS

Course Code: MBC-004

Assignment code: MBC-004/TMA/2026

Maximum marks: 100

Note: Attempt all questions. The marks for each question are indicated against it.

Write the answers in your own words; do not copy from the course material.

PART-(A)

1. How does an enzyme assay work? Explain its different types.

2. Distinguish between the following:

i) Continuous flow and Stopped flow formats in enzyme kinetics

ii) Sequential and Non-Sequential Substrate enzyme reactions

3. Derive Michaelis Menten equation for a non-competitive inhibitor.

4. Explain the following:

i) Transition State Theory

ii) Strain and Distortion theory of enzyme catalysis

5. Explain the IUPAC system of enzyme classification.

PART- (B)

6. Describe the concept of enzyme engineering.

7. What are proteolytic enzymes? Give a brief account of some of them.

8. Discuss the origin of Isozymes and their nomenclature.

9. (a) Describe the role of multienzyme complexes as regulatory enzymes.

(b) Explain application of enzyme in fruit juice and wine industry.

10. Discuss the methods of enzyme purification based on size or mass.

 

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